Carin Thomas (Chemistry)
Mouse liver cells have been utilized in pharmaceutical industry for their vital role of determining the toxicity of chemicals that may be used as novel treatments in disease. Five newly synthesized organic compounds, obtained from Dr. Timothy Beng’s research team (CWU Chemistry), were used as five different xenobiotics to be tested for their toxicity to mammalian cells in vitro. Vector transfected Hepa-V mouse liver cells were grown in 25 cm2 flasks using a complete media solution containing DMEM/F12, 10% Nu-Serum and 1% Penicillin-Streptomycin. When cell confluency reached 80%, the cells were exposed to different treatments including: complete media (no xenobiotics or DMSO), vehicle control (0.5% v/v DMSO), positive control (10% v/v DMSO), or the appropriate xenobiotics (0.1 µg/µL in 0.5% v/v DMSO) for 24 hours. Using the WST-8 assay and the Synergy 2 instrument, cell viability was determined through measuring absorbances at 450nm. The results showed that cell viability in the positive control decreased by 70% on average compared to the vehicle control, indicating the WST-8 assay was working properly. The results also suggested that all five organic compounds were toxic to mouse liver cells, decreasing cell viability by 40-80% as compared to the 0.5% v/v DMSO vehicle control.
Keywords: TOXICITY TESTING, NOVEL TREATMENTS, TOXICITY OF NEWLY SYNTHESIZED ORGANIC COMPOUNDS