Model for Neuronal Growth Cone Guidance Using Optical Signals

Author(s)

Desiree Ramirez

Faculty Mentor(s)

Erin Craig (Physics)

Abstract

During neural development, a structure at the tip of a growing axon called the growth cone detects extrinsic guidance cues in order to advance the axon to the correct synaptic target. Several experimental studies have shown that near infrared (NIR) lasers can change the direction of growth cone motility and axonal outgrowth, with the potential to be used for neural repair once the mechanism for optical guidance of growth cone motility is better understood. Here, we develop a computational model of the growth cone turning mechanism in response to a NIR laser, based on the hypothesis that the laser induces asymmetric growth of an intracellular network of filaments called f-actin. To test our hypothesis, we developed a computational framework in MATLAB to simulate growth cone motility in response to a focused laser beam applied to one side of the growth cone. Our simulations predict changes in the shape of the growth cone that precede growth cone turning toward the site of the laser. The framework we established for predicting growth cone motility for different laser placement strategies can be used as a tool for planning experimental tests. Detailed understanding of NIR laser guidance of growth cones could contribute to the development of minimally invasive techniques for neural repair.

Keywords: Biophysics, Neuroscience, Growth Cones

Presentation

4 thoughts on “Model for Neuronal Growth Cone Guidance Using Optical Signals”

  1. Hi Desiree! Great job on your presentation. I have three questions:
    1) Do scientists know why g-actin is attracted to a NIR laser?
    2) How do you use the g-actin distribution and/or potential energy well to determine the rate of motion on the leading edge of the growth cone?
    3) What is the next step in the further development of the model?

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